Immediately after that, 1 volume of quit mix resolution was included and rocked for about two hrs, centrifuged and the optical density of the solubilized formazan was determined spectrophotometrically measuring the absorbance at 550 nm
SK-N-MC cells had been developed onto 22 mm x 50 mm glass coverslips positioned in a a hundred mm petri-dish at a concentration of 1 x 106 in 8 ml…