nin.12024

Desert Hedgehog/Dhh Antibody (RM0087-7C8) Summary

    Immunogen
    Mouse recombinant dhh C-terminal fragment
    Specificity
    This was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse recombinant protein of dhh C-terminal fragment.
    Isotype
    IgG2
    Clonality
    Monoclonal
    Host
    Rat
    Gene
    DHH
    Purity
    Protein G purified
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Applications/Dilutions

    Dilutions
        Western Blot 1:500-1:1000
    Application Notes
    This antibody detects mouse dhh C-terminal polypeptides, but not Nterminal one in Western blotting.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Buffer
    A 0.2 um filtered solution in PBS
    Preservative
    No Preservative
    Concentration
    LYOPH
    Purity
    Protein G purified
    Reconstitution Instructions
    Reconstitute with 0.2 ml sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Desert Hedgehog/Dhh Antibody (RM0087-7C8)

      desert hedgehog (Drosophila) homolog
      desert hedgehog homolog
      desert hedgehog protein
      Desert Hedgehog
      DHH
      HHG-3MGC35145

Background

This gene encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N terminal portion is soluble and contains the signalling activity while the C terminal portion is involved in precursor processing. More importantly, the C terminal product covalently attaches a cholesterol moiety to the N terminal product, restricting the N terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

GSK3326595

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