Mals.Guex-Crosier, Wittwer, RobergeInjection of CINC in the eye The chemotactic effect of a direct intraocular injection of CINC was evaluated. When CINC was injected in the anterior chamber with the eye of naive rats there was a important influx of cells into the aqueous humour. By Giemsa stain the cells have been almost exclusively PMNs (not shown). The imply cell count within the CINC injected eyes (n=5) was of 630 (213) cells/pl compared with 31 (eight) cells/4l within the eye injected with PBS alone (p=0.047) (Fig three). CINC had no impact on protein exudation. The imply protein concentration was of 1.46 (0.17) mg/ml inside the CINC injected eyes and 1.36 (0.16) mg/ml in the handle eyes (p=0.59) (Fig 3). In naive non-injected rats, the imply protein concentration was 1.41 (0.08) mg/ml.Discussion The study of animal models of endotoxic shock as well as the injection of low doses of endotoxin in human volunteers helped comprehend the inflammatory cascade triggered by LPS.20 21 Inside the blood, the LPS molecule attaches to a carrier protein (LBP), and within this complex kind interacts together with the CD14 cell surface receptor to activate monocyte.22 23 TNF-ax and IL-15 are released, causing a cardiovascular shock.20 2425 TNF-a may be detected in circula- Yan Guex-Crosier was supported by a grant from the Swiss and from tion half an hour immediately after LPS injection and the National Fund for young Researchers Moffat). the Societe Academique Vaudoise (Fondation Fern 26-28 I-1 We would prefer to thank Dr Karen Sokolowski and Dr Marc level peaks 30 minutes later.24 for offering the 1B6 Bree from appears shortly following TNF-a and features a antibody andRepliGen Corporation his helpful ideas. Dr Terrence Knisely for synergistic effect on the production of other inflammatory cytokines for instance IL-6 and Martin MA, Silverman HJ. Gram-negative sepsis and also the adult respiratory distress syndrome. Clin Infect Dis IL-8.24 25 The circulating array of cytokines 1992;14:1213-28. induced by LPS is in all probability responsible for the two Bayston KF. Bacterial endotoxin and current ideas within the diagnosis and treatment ofendotoxaemia.JMedMicroearly rupture from the blood-aqueous barrier that biol 1990;31:73-83. we observed in EIU. Throughout the early phase of 3 Fischer E, Van Zee KJ, Marano MA, Rock CS, Kenney JS, Poutsiaka DD, et al. Interleukin-I receptor antagonist in EIU, the passive entry on the inflammatory experimental inflammation and in human illness. Blood cytokines TNF-a and I-1 from the circula1992;79: 196-200. JA, Wolff SM. Anticytokine stratetion most likely induces the intraocular produc- 4 Dinarello CA, Gelfand the systemic inflammatory response gies within the remedy of tion of things including CINC by ocular tissue syndrome.JAMA 1993;269: 1829-35. cells. Indeed the messenger RNA of several 5 Kuhns DB, Alvord G, Gallin JI. Improved circulating cytokines, cytokine antagonists, and E-selectin immediately after intravecytokines has been detected within the eye in EIU.29 nous administration of endotoxin in humans. J Infect Dis 1995;171:145-52. The intraocular injection of TNF-a was shown 6 Fong Y, Moldawer LL, Marano M, Wei H, Tatter SB, to induce IL-6 production in the eye.’0 In addiClarick RH, et al. Desmocollin-1 Proteins Biological Activity Endotoxinemia elicits increased circulating B2-IFN/IL-6 in man.YImmunol 1989;142:2321-4. tion, ocular specific cells in culture were capable to 7 Bozza PT, Castro-Faria-Neto HC, Silva AR, Larangeira AP, make inflammatory mediators.”-” A large Silva PMR, Martins MA, et al. Lipopolysaccharideinduced pleural Cadherin-9 Proteins Molecular Weight neutrophil accumulation d.