He onset of various ageassociated illnesses and chemotherapy induced premature ageing. Solutions: As a way to understand how senescent cells that accumulate inside organisms with age negatively influence on organ and tissue function, we’ve characterized senescent cell derived extracellular vesicles (EVs) and their miRNA cargo and their functional part within the context of cellular and organismal ageing, specifically on how EV derived miRNAs influence differentiation and proliferation of skin keratinocytes and mesenchymal stem cells. Final results: We identified EVs and circulating miRNAs as bona fide members of your senescence connected secretory phenotype (SASP) which can be transferred from senescent cells to their microenvironment or even the systemic environment. Upon uptake, recipient cells alter their behaviour, like changes in osteogenic differentiation of mesenchymal stem cells, in wound healing of skin keratinocytes or apoptotic behaviour of skin fibroblasts. Particularly inside the context of osteogenic differentiation, we were additional able to show that circulating miRNAs are prognostic biomarkers of osteoporotic fracture danger. Summary/Conclusion: In summary, we present proof on the significance of certain miRNAs and highlight their prospective use as biomarkers of VRK Serine/Threonine Kinase 1 Proteins Biological Activity ageing and age-associated ailments, or even as therapeutic tools and targets to stop age-associated diseases. Funding: This study was funded by Christian Doppler Gesellschaft, FWF, EU FP7 SYBIL, EU FP7 Frailomic.PS06.13 = OWP1.Prostate cancer-derived extracellular vesicles facilitate osteoclast fusion and differentiation via enhancing filopodia formation in osteoclast precursorsSaturday, 05 MayPS07: EVs in Tumor Metastasis Chairs: Takahiro Ochiya; Carla Oliveira Location: Exhibit Hall 17:158:PS07.Extracellular vesicles in an in vivo method for macrophage migration Karen Linnemannstoens; Leonie Witte; Julia Christina Gross University Healthcare Center Cathepsin G Proteins Gene ID Goettingen, Goettingen, GermanyBackground: Cell migration can be a polarized cellular process in which the protruding leading edge opposes a retracting trailing edge. EVs control directionally persistent cell migration by developing an autocrine nearby gradient. This calls for polarized delivery of MVBs to the plasma membrane and subsequent polarized secretion. Though the majority of the studies in cell culture concentrate on migratory phenotypes, EV studies in developmental/physiological signalling have largely been carried out in polarized epithelia like imaginal discs. The query is no matter if the exact same cellular machineries direct the selective sorting of cargo into diverse vesicles which are then secreted apically vs. basally or in the leading vs. trailing edge respectively. Procedures: To understand this complicated course of action within a multicellular organism, we study EV biogenesis and polarized secretion inside the model of migrating Drosophila pupal haemocytes, which are elements from the haemolymph and constitute blood cells and macrophages in flies. Whereas isolation of EVs from cell culture supernatants and human serum are established, neither the presence nor function of secreted EVs in haemolymph has been studied so far. We established a technique to purify EVs from haemolymph by differential centrifugation and analysed the resulting pellets by quite a few approaches. We generated several EV reporter fly lines expressing fluorescently labelled haemocytes and EV marker. These lines let to visualize each haemocytes and vesicles within the cells and characterize their.