j.aap.2016.03.002

APRIL/TNFSF13 Antibody (670840) [Alexa Fluor® 700] Summary

    Immunogen
    Human embryonic kidney cell line HEK293EBNA-derived recombinant human APRIL/TNFSF13
    Ala105-Leu250
    Accession # O75888
    Specificity
    Detects human APRIL/TNFSF13 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinantmouse APRIL, recombinant human (rh) BAFF, rhEDA-A2, rhEDA, rhFas Ligand,rhLIGHT, rhLT a1/b2, rhLT a2/b1, rhOX40 Ligand, rhTNF alpha, rhTRAIL, rhTRANCE,rh4-1BB Ligand, rhVEGI, or rhGITR Ligand is observed.
    Isotype
    IgG2b
    Clonality
    Monoclonal
    Host
    Mouse
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Applications/Dilutions

    Dilutions
        Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

    Storage
    Store the unopened product at 2 – 8 °C. Do not use past expiration date.
    Buffer
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APRIL/TNFSF13 Antibody (670840) [Alexa Fluor® 700]

      A proliferation-inducing ligand
      APRIL
      APRILFLJ57090
      CD256 antigen
      CD256
      TALL2
      TALL-2
      TNF- and APOL-related leukocyte expressed ligand 2
      TNF-related death ligand 1
      TNFSF13
      TRDL1
      TRDL-1
      tumor necrosis factor (ligand) superfamily, member 13
      tumor necrosis factor ligand superfamily member 13
      tumor necrosis factor-like protein ZTNF2
      tumor necrosis factor-related death ligand-1
      ZTNF2

Background

APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

product targets : Immunology_Inflammation inhibitors

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