c approach, dicarboxylic acid metabolic method, and alpha amino acid biosynthetic course of action.two.4. Interaction Networks and Biosynthetic Pathways inside DEGs and DEPs In the outcomes from the DEGs having a log2 fold modify three.9, the main biological function categories were discovered to become the leucine catabolic method, L-phenylalanine catabolic process, jasmonic acid biosynthetic procedure, response to higher light intensity, response to hy6 of drogen peroxide, alpha-amino acid catabolic process, response to heat, response to chitin, 15 response to a virus, and response to a temperature stimulus. The interaction network of proteins encoded by drought-tolerance-related DEGs is shown in Figure 2.Plants 2021, 10,Figure 2. Interaction network of proteins encoded by drought-tolerance-related DEGs. The empty nodes represent proteins of an unknown 3D structure, along with the filled nodes signify that some 3D structure is identified or predicted. Edges represent pULK1 Storage & Stability rotein rotein associations (vibrant blue and pink color–known interactions, green and dark blue color–predicted interactions, yellow color–text mining, black color–co-expression). Proteins marked inside the blue square have been substantially connected with OX2 Receptor custom synthesis drought pressure with an adjusted p-value 0.05, and log2 fold alter three.9. Disconnected nodes or proteins not connected towards the most important network have been hidden inside the network.The evaluation of enriched metabolic pathways (Figure 4) showed some congruences among transcriptomic and proteomic levels, e.g., tetrapyrrole binding and photosynthetic response generally. On the other side, transcriptomic response was exceptional inside the protein folding/refolding pathway, and in amylase activity. To the contrary, proteomic pathways have been uniquely enriched in responses to oxidative strain, alkaloid metabolism, and others.Plants 2021, 10, 1878 Plants 2021, ten, x FOR PEER REVIEW7 of 15 eight ofFigure three. Co-expression network of drought-tolerance-related DEPs. The empty nodes represent proteins of an unknown Figure 3. Co-expression network of drought-tolerance-related DEPs. The empty nodes represent proteins of an unknown 3D structure, plus the filled nodes signify that some 3D structure is recognized or predicted. Edges represent protein rotein 3D structure, as well as the filled nodes signify that some 3D structure is identified or predicted. Edges represent protein rotein associations (vibrant blue and pink–known interactions, green and dark blue–predicted interactions, yellow–text minassociations (vibrant blue and pink–known interactions, green and dark blue–predicted interactions, yellow–text mining, ing, black–co-expression, multicolor–protein homology). Proteins marked within the red circle have been those with an adjusted black–co-expression, marked inside the blue homology). Proteins marked inside the red circle have been those with an adjusted p-value 0.05. Proteins multicolor–proteinsquare are associated with drought anxiety. Disconnected nodes or proteins not p-value for the principal network in the blue square network. connected0.05. Proteins markedwere hidden in the are related with drought pressure. Disconnected nodes or proteins not connected to the principal network were hidden within the network.Plants 2021, ten, 1878 Plants 2021, ten, x FOR PEER REVIEW8 of 15 9 ofFigure Enriched metabolic pathways below the drought condition in germinating Papaver somniferum plants. A hierarchiFigure 4.4. Enriched metabolic pathways below the drought situation in germinating Papaver somniferum plants. A hierarchical clustering tree summarizin