tissue dehydration, adaptive immune response, protein binding, activation of connected enzymes, migration of immune cells, and so on. In this descriptive study, our findings revealed the adjustments inside the host transcriptome expression profile even though feeding an antibioticfree eating plan and recommended that feeding an antibiotic-free diet regime alters the host’s metabolic network and the expression of antiviral proteins. Abstract: China officially promulgated the announcement of banning the usage of antibiotics inside the animal sector in 2020. However, the prohibition of antibiotics in the animal business would cut down the feed conversion rate and boost the mortality of animals. As a way to get information regarding the TLR8 list pathogenesis and host immune response of rabbits with diarrhea just after becoming fed an antibioticfree diet program, we 1st analyzed the intestinal tissue sections of rabbits. Secondly, the gene expression RelA/p65 Formulation variations of rabbit intestinal segments have been analyzed by high-throughput sequencing. Our analysis identified 168, 593, 2069, 334, 321, and 1423 DEGs within the comparison groups S_Z (the duodenum of healthier rabbits) vs. S_B (diarrhea in the duodenum of rabbits), K_Z (healthful rabbit jejunum) vs. K_B (rabbits with diarrhea within the jejunum), H_Z (wholesome rabbit ileum) vs. H_B (rabbits with diarrhea inside the ileum), M_Z (healthy cecum of rabbits) vs. M_B (rabbits with diarrhea in the cecum), J_Z (healthy rabbit colon) vs. J_B (colon of rabbits with diarrhea), and Z_Z (healthy rabbit rectum) vs. Z_B (rectum of rabbits with diarrhea), respectively. The reproducibility and repeatability of your benefits have been validated by RT-qPCR. Enrichment analyses of GO annotations and KEGG pathways revealed the host DEGs which can be potentially connected to acute inflammation, pressure response, tissue dehydration, adaptive immune response, protein binding, activation of related enzymes, migration of immune cells, and so on. In this descriptive study, our findings revealed the changes in the host transcriptome expression profile soon after feeding an antibiotic-free eating plan and recommended that feeding an antibiotic-free diet plan alters the host’s metabolic network and the expression of antiviral proteins, which provides a theoretical basis for further study on the immune response of animals fed an antibiotic-free diet.Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is definitely an open access short article distributed under the terms and situations with the Creative Commons Attribution (CC BY) license ( creativecommons.org/licenses/by/ four.0/).Animals 2021, 11, 2994. doi.org/10.3390/animdpi/journal/animalsAnimals 2021, 11,two ofKeywords: transcriptomic analysis; no-antibiotic eating plan; diarrhea; rabbit1. Introduction Since the initially use of antibiotics in piglets and chickens in 1949, it has been located that feeding diets containing antibiotics could sustain animal intestinal overall health and decrease livestock mortality [1,2]. However, feeding an antibiotic diet program would steadily weaken the immune potential of animals, causing animal resistance to antibiotics and forming a vicious circle, and leading to human wellness troubles brought on by antibiotic residues in meat [3]. Thus, the animal industry and also the customer marketplace have been increasingly calling for the abolition of the use of feed antibiotics [4,5]. For example, Sweden, the European Union, and China banned the usage of antibiotics in