S with multiple myeloma Tomohiro Umezu1, Satoshi Satoshi2, Seiichiro Yoshizawa1, Kazuma Ohyashiki1 and Junko H. Ohyashiki1Thursday May 18,Department of Haematology, Tokyo Health-related University, Tokyo, Japan; Institute of Health-related Science, Tokyo Medical University, Tokyo, JapanIntroduction: Multiple myeloma (MM) is refractory haematologic malignancy. Bone marrow stromal cells (BMSCs) interact with MM cells in the bone marrow (BM), as well as develop a permissive Adenosine Deaminase drug microenvironment for MM cell growth and survival. Current proof indicated that exosome-mediated MM cell-BMSC communication plays an important role within the MM microenvironment. In this study, we investigated the biological property from the exosomes and exosomal miRNAs derived from BMSCs, aiming to establish the emerging techniques to target MM microenvironment to prevent tumour growth and spread. Solutions: BM samples have been obtained from MM individuals, and BMSCs (mmBMSCs) had been isolated applying the classical plastic adhesion approach. BMSCs from healthier donors (normalBMSCs) had been bought from Lonza Inc. The exosomes were isolated from conditioned medium ofBMSCs making use of Exoquick-TC Reagent (System Biosciences). Cellular and exosomal miRNA profiling was done using a TaqMan low-density array (Applied Biosystems). For functional analysis, the miRNA mimic (Ambion) was overexpressed in BMSCs, and WST-8 (Dojindo) and Caspase-Glo assays (Promega) were performed to ascertain the influence on cell proliferation and apoptosis, respectively. Final results: We located that exosomal miRNA expression was unique involving mmBMSCs and normalBMSCs. We found that miR-10a was considerably upregulated within the exosomes derived mmBMSCs, when the expression of miR-10a was low in mmBMSCs. We hypothesised that low expression of cellular miR-10a could be essential for survival of mmBMSCs, hence the miR-10a packaged into exosomes could possibly be released in to the extracellular space. Of note is that overexpression of miR-10a inhibited proliferation, and promoted apoptosis in mmBMSCs. Conclusion: Our final results deliver the possibility that the inhibition of exosome release may induce mmBMSC apoptosis.Scientific System ISEVPoster Session PT11 EVs as well as the Immune Method Chairs: TBD and Susanne van der GreinPT11.In vivo analysis in the possible of exosomes isolated from menstrual blood-derived mesenchymal stem cells in regeneration of insulinproducing cells in diabetic kind 1 animal model Elahe Mahdipour, Zahra Salmasi and Nona Sabeti Department of Medical Biotechnology, College of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran5:15:30 p.m.Introduction: Diabetes type 1 is characterised by the lack of insulin production as a result of degeneration of insulin-producing beta cells within the pancreas. The autoimmune response H1 Receptor Formulation against beta cells may be the primary explanation for this disease; hence, any approaches that support immune response regulation may be valuable. Research have shown the effectiveness of mesenchymal stem cells (MSCs) in regulation of T cell response and pancreatic islet repair. Nonetheless, application of MSCs accompanies the cell therapy security challenge. The unknown fate of injected stem cells is among the main safety issues concerning stem cell therapies; hence, in this study we’ve used the exosomal secretome of MSCs to regenerate insulin-producing cells. Strategies: MSCs were isolated from menstrual blood as a rich and noninvasive supply of MSCs. Exosomes had been isolated and characterised making use of western blot and AFM, TEM techn.