Ighbouring cells against pathogenic signals. Solutions: Exosomes were isolated by ultracentrifugation from bio-fluids (urine, synovial fluid) of people with microbial infections and from healthier controls. Exosomes were filtered applying 0.22 filter and total RNA was isolated employing miRCURY RNA isolation kit (Exiqon, DK). Differential expression of TLR-mRNA content against each and every infection was assessed by actual time PCR. In addition, B and T-cells have been stimulated in vitro and exosomes were examined for TLR-mRNA as when compared with exosomes from non-stimulated cells. Benefits: As in comparison to healthier subjects the TLRs 5, six, 7 and 9 were strongly upregulated in exosomes from urine of E. coli infected patients. Wherease, TLRs two and 9 were downregulated from arthritic patient derived synovial fluid exosomes as in comparison with wholesome people. TLRs in exosomes from stimulated B-cells had been upregulated, whereas these from T-cells were downregulated as in comparison with exosomes from non-stimulated cells. This indicates the secretion of differentially expressed TLR-mRNA from infected people too as stimulated cells as when compared with healthier donors and non-stimulated cells. Conclusion: Secretion of TLR-mRNA by way of exosomes is actually a potent way of communicating defensive messages against pathogen invasion. This indicates the roles of exosomes in mediating host athogen interactions by shuttling TLR messages which raises the possibility to use exosomes as potential biomarkers aginsat infections. Even so, furthers research could be required to elucidate underlying mechanisms.PT08.Extracellular SIRT3 Storage & Stability vesicles from Staphylococcus mGluR3 Synonyms aureus and Staphylococcus epidermidis are related with tiny RNA Forugh Vazirisani1,2, Margarita Trobos1,2, Furqan A. Shah1, Peter Thomsen1,two and Karin Ekstr 1,2 Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; 2BIOMATCELL, VINN Excellence Centre of Biomaterials and Cell Therapy, Gothenburg, SwedenPT08.Secretion of Toll-like receptor mRNAs through exosomes: a probable way of communicating messages against pathogens Muhammad Nawaz1,two, Jessica Wahlgren1, Luisa Statello1, Marco Maugeri1, Alexandros Papadimitrio1 and Hadi ValadiUniversity of Gothenburg, Gothenburg, Sweden; 2University of Sao Paulo, Sao Paulo, BrazilIntroduction: Toll-like receptors (TLRs) are transmembrane receptors generally expressed in sentinel cells and are thought to play an essential role inside the recognition of invading microorganisms, such as viruses,Introduction: Delivery of bacterial elements, proteins, and toxins by extracellular vesicles (EVs) from Gram-positive bacteria can have an effect on modulation from the host immune response and cell death. Nevertheless, small is known if genetic materials are connected with these EVs. The aim in the present study was to investigate whether EVs from Staphylococcus aureus and S. epidermidis contain RNA, and in that case, irrespective of whether the RNA content and profile differ among EVs isolated from distinctive bacterial species and strains. Strategies: EVs have been isolated from S. aureus ATCC 25923, S. epidermidis ATCC 35984, and two clinical strains S. aureus 64516 and S. epidermidis 64518 isolated from osteomyelitis related with orthopaedic implants, by filtration and centrifugation methods. EVs were characterised by Western blot, Nanosight and electron microscopy. RNA was extracted, detected and quantified by Ribogreen fluorescence and Bioanalyzer. RNase therapy was used to confirm that the dete.