Y regulate of cell growth and transformation. Associated with intracellular signal CDK5 Inhibitor Biological Activity transduction mediated by cytokines and growth elements. H1 Receptor Inhibitor custom synthesis Signals from tyrosine kinase receptors and tiny GTPases for the actin cytoskeleton. Acts as GTPase-activating protein for RAB27A. OCLN N 24.6 16.six twelve.4 eight.six OCLN C 71.1 ND eleven.1 five.4 CLD4 N ND ND (three.4) (2.9) ZO-1 N 17.6 ND eight.5 four.8 ZO-1 C 51.six ND two.six two.4 ECAD 22.1 ND 29.two ND Reference7.9.twelve.NDNDND6.three.13.NDND2.Muscarinic acetylcholine receptor M4 Segment polarity protein dishevelled homolog DVL-2 Segment polarity protein dishevelled homolog DVL-3 Merlin Protein FAM83B SH2 domain-containing adapter protein B Tubby-related protein three Fibroblast development aspect receptor substrate 2 G-protein coupled receptor relatives C group 5 member C Abl interactor5.ND3.NDNDND4.five.ND8.2.2.4.5.ND6.NDND73994955 570950344.3 4.3 3.three.6 (0.3) three.ND 6.2 (2.five)ND 0.3 NDND ND NDND (0.seven) ND [116]739977963.eight 3.6.seven 3.6.eight one.ND NDND NDND (0.7)(2.6)ND3.NDNDND345793416 73948705 345793418(2.one)two.10.0.NDNDSignal transducing adapter molecule one Wiskott-Aldrich syndrome protein member of the family two TBC1 domain family member 10A(1.three)2.NDNDND5.(1.1)(0.four)four.NDNDND(0.9)(0.seven)four.NDNDND (Continued)PLOS One particular DOI:ten.1371/journal.pone.0117074 March 19,seven /Signaling and Trafficking Networks Surround Occludin and Claudin-Table 2. (Continued) Accession 345792359 Title Epidermal growth component receptor kinase substrate eight V-crk sarcoma virus CT10 oncogene homolog (avian)-like GRB2-associatedbinding protein 1 Cytoplasmic protein NCK1 Switch-associated protein 70 BRCA1-associated protein Localization/Function-Signaling May possibly play a position in membrane ruffling and remodeling of the actin cytoskeleton. Adapter protein involved with various signaling pathways. Signaling adapter triggered by receptortype kinases. Acts in FGFR1 signaling. Role in transcriptional activation in response to activated Ras. Function in cell adhesion and migration through ephrin. Mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein Negatively regulates MAP kinase activation. Also acts like a Ras responsive E3 ubiquitin ligase. Act like a cytoplasmic retention protein. Inhibits TNF-induced apoptosis. Perform a position from the pro-inflammatory cytokine IL-1 signaling. Associated with EGFR signaling. Anchoring protein that mediates the subcellular compartments of PKA and PKC. Might be part of the cortical cytoskeleton. Vesicle transport, signaling and tumor progression. EPCAM family member. May possibly function like a development issue receptor. Associated with amino acid sensing and activation of mTORC1. Promotes cell development in response to development factors. OCLN N ND OCLN C 15.0 CLD4 N 22.five ZO-1 N ND ZO-1 C ND ECAD (0.9) ReferenceND13.ND14.17.ND345784160ND ND6.five five.ND NDND NDND ND1.one NDND3.NDNDNDNDND2.NDNDNDNDTax1-binding protein 3like Coiled-coil domaincontaining protein 50 A-kinase anchor proteinND2.NDNDND1.345796461ND ND1.9 NDND eight.ND NDND ND1.two ND73992442Tumor protein D54 isoform one Tumor-associated calcium signal transducer 2 Ragulator complicated protein LAMTORND NDND ND5.five 5.ND NDND ND3.2 ND [63]NDND4.NDNDNDdoi:ten.1371/journal.pone.0117074.tover the cell [10]. Given the overlapping distribution with ZO-1 also as expression about the lateral cell membrane, we would count on proteins biotinylated from the fusion proteins to contain TJ proteins also recognized by ZO-1 at the same time as novel pertinent lateral plasma membrane proteins and trafficking proteins.Coomassie-stained Protein Gels of Samples from MDCK II-cells E.