Significance is indicated as in Fig. 2C. , 0.001. C, endocytic markers. Cells expressing the indicated RFP fusion proteins (red) were incubated in medium containing 20 M PepS-DyLight 488 and imaged in the indicated time points by in vivo confocal microscopy. Scale bar, 20 m. EIPA.which suggests a compensation effect on endocytic uptake by means of clathrin-independent endocytosis, for instance pinocytosis and macropinocytosis. All round, the partial and compensatory effects triggered by the inhibitors indicate that the peptide was internalized as bulk in the fluid phase Cadherin-24 Proteins Biological Activity Although this additional experiment doesn’t resolve the specific biophysical things determining aggregate uptake, these final results recommend that aggregate size plays an essential modulating role inside the mode of aggregate uptake by cells.JANUARY two, 2015 VOLUME 290 NUMBERPepL but Not PepS Internalization Needs Hsp70 and Is HSF1-dependent–Because phagocytosis is often a specifi.