s13104-016-1986-6

RPTOR Antibody (514208) [Alexa Fluor® 700] Summary

    Immunogen
    E. coli-derived recombinant human RPTOR
    Lys77-Gln230
    Accession # Q8N122
    Specificity
    Detects human and mouse RPTOR in Western blots.
    Isotype
    IgG2b
    Clonality
    Monoclonal
    Host
    Mouse
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Applications/Dilutions

    Dilutions
        Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

    Storage
    Store the unopened product at 2 – 8 °C. Do not use past expiration date.
    Buffer
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RPTOR Antibody (514208) [Alexa Fluor® 700]

      KIAA1303
      KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
      KOG1
      Mip1
      p150 target of rapamycin (TOR)-scaffold protein
      Raptor
      regulatory associated protein of MTOR, complex 1
      regulatory-associated protein of mTOR
      RPTOR

Background

RPTOR (Raptor) is a 150 kDa component of the cytosolic mammalian target of Rapamycin complex 1 (mTORC1) which also contains mTOR and GBL proteins. mTORC1 plays a dominant role in cell cycle regulation in response to metabolic conditions. The interaction of RPTOR with the kinase mTOR is stabilized under conditions of nutrient deprivation and energy stress, leading to inhibition of mTOR and cell cycle arrest. RPTOR contains multiple Ser and Thr residues whose phosphorylation regulates the activation status of mTOR. RPTOR is critical for the response of skeletal muscle and adipose tissue to insulin. It contains three RNC blocks (aa 48-511), three HEAT repeats (aa 550-667), and seven C-terminal WD40 domains (aa 1020-1335). Within aa 77-230, human RPTOR shares 100% aa sequence identity with mouse and rat RPTOR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

product targets : BCRP inhibitors

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